2A1T
Structure of the human MCAD:ETF E165betaA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8123 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.184, 100.659, 244.384 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.800 |
| R-factor | 0.2025 |
| Rwork | 0.199 |
| R-free | 0.26860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t9g |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.614 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0011) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.097 | 0.370 |
| Number of reflections | 55260 | |
| <I/σ(I)> | 8.4 | 2.1 |
| Completeness [%] | 89.2 | 82 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 5000MME, 50mM sodium thiocyanate, sodium cacodylate 0.1M, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
