28LU

Structure of the Chlamydomonas reinhardtii chlororibosome with factor pY

これはPDB形式変換不可エントリーです。

28LU の概要

• エントリーDOI: 10.2210/pdb28lu/pdb
• EMDBエントリー: 56602
• 分子名称: Ribosomal protein S1 homologue, Small ribosomal subunit protein uS14c, 30S ribosomal protein S15, ... (62 entities in total)
• 機能のキーワード: chloroplast, ribosome, translation, chlororibosome
• 由来する生物種: Chlamydomonas reinhardtii; 詳細
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 2670767.53

構造登録者

Waltz, F.,Kater, L.,Engel, B.D. (登録日: 2026-02-05, 公開日: 2026-04-01)

主引用文献

Waltz, F.,Lehner, P.A.,Van der Stappen, P.,Kater, L.,Pfeffer, S.,Engel, B.D.
Chloroplast-encoded small subunit extensions reshape the Chlamydomonas chlororibosome.
Biorxiv, 2026

PubMed Abstract: Chloroplast ribosomes (chlororibosomes) synthesize the core protein components of the photosynthetic apparatus, yet their structural diversity outside flowering plants remains largely unexplored. Here, we combine in situ cryo-electron tomography (cryo-ET) with single-particle cryo-electron microscopy (cryo-EM) to determine the structure of the chlororibosome from the unicellular green alga . Subtomogram averaging of chlororibosomes in their native environment, resolved to ∼5 Å resolution and in distinct translational states, reveals particles both free in the stroma and loosely tethered to thylakoid membranes. These reconstructions uncover an additional "arm" domain on the small subunit. High-resolution single-particle reconstruction of isolated chlororibosomes to ∼2.5 Å, in states bound either to the inhibitory translation factor pY or to a nascent chain-linked P-site tRNA, reveals that this domain is built primarily from extensive chloroplast-encoded insertions and extensions of conserved small subunit proteins, supported by chlororibosome-specific ribosomal proteins. The arm domain is located around the mRNA entry and exit channels, suggesting a role in stabilizing the mRNA trajectory through the small subunit and organizing chloroplast polysomes. Together, these data reveal unexpected structural variation of algal chlororibosomes and suggest that chloroplast translation has diversified substantially even among relatively closely related photosynthetic lineages.

PubMed: 41727112
DOI: 10.64898/2026.02.07.704542

実験手法

ELECTRON MICROSCOPY (2.6 Å)