1YKB

Crystal Structure of Insect Cell Expressed IL-22

Summary for 1YKB

• Entry DOI: 10.2210/pdb1ykb/pdb
• Descriptor: PROTEIN (Interleukin-22), 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: interleukin, cytokine, il-22, glycosylation
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: Q9GZX6
• Total number of polymer chains: 6
• Total formula weight: 102244.08

Authors

Xu, T.,Logsdon, N.J.,Walter, M.R. (deposition date: 2005-01-17, release date: 2005-07-12, Last modification date: 2024-10-30)

Primary citation

Xu, T.,Logsdon, N.J.,Walter, M.R.
Structure of insect-cell-derived IL-22.
Acta Crystallogr.,Sect.D, 61:942-950, 2005

PubMed Abstract: The crystal structure of interleukin-22 expressed in Drosophila melanogaster S2 cells (IL-22(Dm)) has been determined at 2.6 A resolution. IL-22(Dm) crystals contain six molecules in the asymmetric unit. Comparison of IL-22(Dm) and IL-22(Ec) (interleukin-22 produced in Escherichia coli) structures reveals that N-linked glycosylation causes only minor structural changes to the cytokine. However, 1-4 A main-chain differences are observed between the six IL-22(Dm) monomers at regions corresponding to the IL-22R1 and IL-10R2 binding sites. The structure of the carbohydrate and the conformational variation of IL22(Dm) provide new insights into IL-22 receptor recognition.

PubMed: 15983417
DOI: 10.1107/S0907444905009601

Experimental method

X-RAY DIFFRACTION (2.6 Å)