1YKB
Crystal Structure of Insect Cell Expressed IL-22
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Wavelength(s) | 1.255 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.877, 62.227, 139.524 |
Unit cell angles | 90.00, 91.35, 90.00 |
Refinement procedure
Resolution | 19.930 - 2.600 |
Rwork | 0.232 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | BEAST |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.950 |
High resolution limit [Å] | 2.500 |
Number of reflections | 33239 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 310 | pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 310K |