1XSP

Crystal Structure of human DNA polymerase lambda in complex with nicked DNA and pyrophosphate

1XSP の概要

• エントリーDOI: 10.2210/pdb1xsp/pdb
• 関連するPDBエントリー: 1XSL 1XSN
• 分子名称: 5'-D(*CP*GP*GP*CP*CP*GP*TP*AP*CP*TP*G)-3', 5'-D(*CP*AP*GP*TP*AP*CP*G)-3', 5'-D(P*GP*CP*CP*G)-3', ... (7 entities in total)
• 機能のキーワード: dna polymerase lambda, protein-dna complex, helix-hairpin-helix, transferase-dna complex, transferase/dna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus : Q9UGP5
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 44382.05

構造登録者

Garcia-Diaz, M.,Bebenek, K.,Krahn, J.M.,Kunkel, T.A.,Pedersen, L.C. (登録日: 2004-10-19, 公開日: 2005-01-18, 最終更新日: 2024-02-14)

主引用文献

Garcia-Diaz, M.,Bebenek, K.,Krahn, J.M.,Kunkel, T.A.,Pedersen, L.C.
A closed conformation for the Pol lambda catalytic cycle.
Nat.Struct.Mol.Biol., 12:97-98, 2005

PubMed Abstract: Pol lambda is a family X member believed to fill short gaps during DNA repair. Here we report crystal structures of Pol lambda representing three steps in filling a single-nucleotide gap. These structures indicate that, unlike other DNA polymerases, Pol lambda does not undergo large subdomain movements during catalysis, and they provide a clear characterization of the geometry and stereochemistry of the in-line nucleotidyl transfer reaction.

PubMed: 15608652
DOI: 10.1038/nsmb876

実験手法

X-RAY DIFFRACTION (2.2 Å)