1W2H

Crystal Structure Of Mycobacterium Tuberculosis Thymidylate Kinase Complexed With Azidothymidine Monophosphate (AZT-MP) (2.0 A Resolution)

Summary for 1W2H

• Entry DOI: 10.2210/pdb1w2h/pdb
• Related: 1G3U 1GSI 1GTV 1MRN 1MRS 1N5I 1N5J 1N5K 1N5L 1W2G
• Descriptor: THYMIDYLATE KINASE TMK, 3'-AZIDO-3'-DEOXYTHYMIDINE-5'-MONOPHOSPHATE, ACETATE ION, ... (4 entities in total)
• Functional Keywords: transferase, mycobacterium tuberculosis, thymidylate kinase, azt, inhibition mechanism
• Biological source: MYCOBACTERIUM TUBERCULOSIS
• Total number of polymer chains: 2
• Total formula weight: 46543.85

Authors

Fioravanti, E.,Adam, V.,Munier-Lehmann, H.,Bourgeois, D. (deposition date: 2004-07-06, release date: 2005-01-12, Last modification date: 2023-12-13)

Primary citation

Fioravanti, E.,Adam, V.,Munier-Lehmann, H.,Bourgeois, D.
The crystal structure of Mycobacterium tuberculosis thymidylate kinase in complex with 3'-azidodeoxythymidine monophosphate suggests a mechanism for competitive inhibition.
Biochemistry, 44:130-137, 2005

PubMed Abstract: Tuberculosis (TB) is the primary cause of mortality among infectious diseases. Mycobacterium tuberculosis thymidylate kinase (TMPK(Mtub)) catalyzes the ATP-dependent phosphorylation of deoxythymidine 5'-monophosphate (dTMP). Essential to DNA replication, this enzyme represents a promising target for developing new drugs against TB, because the configuration of its active site is unique within the TMPK family. Indeed, it has been proposed that, as opposed to other TMPKs, catalysis by TMPK(Mtub) necessitates the transient binding of a magnesium ion coordinating the phosphate acceptor. Moreover, 3'-azidodeoxythymidine monophosphate (AZTMP) is a competitive inhibitor of TMPK(Mtub), whereas it is a substrate for human and other TMPKs. Here, the crystal structures of TMPK(Mtub) in complex with deoxythymidine (dT) and AZTMP were determined to 2.1 and 2.0 A resolution, respectively, and suggest a mechanism for inhibition. The azido group of AZTMP perturbs the induced-fit mechanism normally adopted by the enzyme. Magnesium is prevented from binding, and the resulting electrostatic environment precludes phosphoryl transfer from occurring. Our data provide a model for drug development against tuberculosis.

PubMed: 15628853
DOI: 10.1021/bi0484163

Experimental method

X-RAY DIFFRACTION (2 Å)