1V41

Crystal structure of human PNP complexed with 8-Azaguanine

Summary for 1V41

• Entry DOI: 10.2210/pdb1v41/pdb
• Related: 1V3Q 1V45
• Descriptor: Purine nucleoside phosphorylase, SULFATE ION, 5-AMINO-1H-[1,2,3]TRIAZOLO[4,5-D]PYRIMIDIN-7-OL, ... (4 entities in total)
• Functional Keywords: purine nucleoside phosphorylase, drug design, sybcgritron, 8-azaguanine, transferase
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm, cytoskeleton (By similarity): P00491
• Total number of polymer chains: 1
• Total formula weight: 32590.05

Authors

Dos Santos, D.M.,Canduri, F.,Silva, R.G.,Mendes, M.A.,Basso, L.A.,Palma, M.S.,De Azevedo Jr., W.F.,Santos, D.S. (deposition date: 2003-11-08, release date: 2004-12-14, Last modification date: 2023-10-25)

Primary citation

Canduri, F.,Silva, R.G.,dos Santos, D.M.,Palma, M.S.,Basso, L.A.,Santos, D.S.,de Azevedo Jr., W.F.
Structure of human PNP complexed with ligands.
Acta Crystallogr.,Sect.D, 61:856-862, 2005

PubMed Abstract: Purine nucleoside phosphorylase (PNP) is a key enzyme in the purine-salvage pathway, which allows cells to utilize preformed bases and nucleosides in order to synthesize nucleotides. PNP is specific for purine nucleosides in the beta-configuration and exhibits a strong preference for purines containing a 6-keto group and ribosyl-containing nucleosides relative to the corresponding analogues. PNP was crystallized in complex with ligands and data collection was performed using synchrotron radiation. This work reports the structure of human PNP in complex with guanosine (at 2.80 A resolution), 3'-deoxyguanosine (at 2.86 A resolution) and 8-azaguanine (at 2.85 A resolution). These structures were compared with the PNP-guanine, PNP-inosine and PNP-immucillin-H complexes solved previously.

PubMed: 15983407
DOI: 10.1107/S0907444905005421

Experimental method

X-RAY DIFFRACTION (2.85 Å)