1V41
Crystal structure of human PNP complexed with 8-Azaguanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 104 |
| Detector technology | CCD |
| Collection date | 2003-06-26 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.4310 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 139.280, 139.280, 161.420 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.850 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v2h |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.812 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.496 | 3.010 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.045 | 0.125 |
| Number of reflections | 12929 | |
| Completeness [%] | 91.0 | 90.4 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 298 | AMMONIUM SULFATE, pH 5.30, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
