1UY8

Human Hsp90-alpha with 9-Butyl-8-(3-trimethoxy-benzyl)-9H-purin-6ylamine

1UY8 の概要

• エントリーDOI: 10.2210/pdb1uy8/pdb
• 関連するPDBエントリー: 1BYQ 1OSF 1UY6 1UY7 1UY9 1UYC 1UYD 1UYE 1UYF 1UYG 1UYH 1UYI 1UYK 1UYL 1UYM 1YER 1YES 1YET
• 分子名称: HEAT SHOCK PROTEIN HSP 90-ALPHA, 9-BUTYL-8-(3-METHOXYBENZYL)-9H-PURIN-6-AMINE (3 entities in total)
• 機能のキーワード: hsp90, atpase, pu5, chaperone, atp-binding, heat shock
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cytoplasm: P07900
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26913.16

構造登録者

Wright, L.,Barril, X.,Dymock, B.,Sheridan, L.,Surgenor, A.,Beswick, M.,Drysdale, M.,Collier, A.,Massey, A.,Davies, N.,Fink, A.,Fromont, C.,Aherne, W.,Boxall, K.,Sharp, S.,Workman, P.,Hubbard, R.E. (登録日: 2004-03-02, 公開日: 2004-07-01, 最終更新日: 2023-12-13)

主引用文献

Wright, L.,Barril, X.,Dymock, B.,Sheridan, L.,Surgenor, A.,Beswick, M.,Drysdale, M.,Collier, A.,Massey, A.,Davies, N.,Fink, A.,Fromont, C.,Aherne, W.,Boxall, K.,Sharp, S.,Workman, P.,Hubbard, R.E.
Structure-Activity Relationships in Purine-Based Inhibitor Binding to Hsp90 Isoforms
Chem.Biol., 11:775-, 2004

PubMed Abstract: Inhibition of the ATPase activity of the chaperone protein HSP90 is a potential strategy for treatment of cancers. We have determined structures of the HSP90alpha N-terminal domain complexed with the purine-based inhibitor, PU3, and analogs with enhanced potency both in enzyme and cell-based assays. The compounds induce upregulation of HSP70 and downregulation of the known HSP90 client proteins Raf-1, CDK4, and ErbB2, confirming that the molecules inhibit cell growth by a mechanism dependent on HSP90 inhibition. We have also determined the first structure of the N-terminal domain of HSP90beta, complexed with PU3. The structures allow a detailed rationale to be developed for the observed affinity of the PU3 class of compounds for HSP90 and also provide a structural framework for design of compounds with improved binding affinity and drug-like properties.

PubMed: 15217611
DOI: 10.1016/J.CHEMBIOL.2004.03.033

実験手法

X-RAY DIFFRACTION (1.98 Å)