1U8C

A novel adaptation of the integrin PSI domain revealed from its crystal structure

Summary for 1U8C

• Entry DOI: 10.2210/pdb1u8c/pdb
• Related: 1JV2
• Descriptor: Integrin alpha-V, Integrin beta-3, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• Functional Keywords: psi domain, integrin, vitronectrin receptor, cell adhesion
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 2
• Total formula weight: 186847.78

Authors

Xiong, J.P.,Stehle, T.,Goodman, S.L.,Arnaout, M.A. (deposition date: 2004-08-05, release date: 2004-12-28, Last modification date: 2024-10-16)

Primary citation

Xiong, J.P.,Stehle, T.,Goodman, S.L.,Arnaout, M.A.
A novel adaptation of the integrin PSI domain revealed from its crystal structure.
J.Biol.Chem., 279:40252-40254, 2004

PubMed Abstract: Integrin beta-subunits contain an N-terminal PSI (for plexin-semaphorin-integrin) domain that contributes to integrin activation and harbors the PI(A) alloantigen associated with immune thrombocytopenias and susceptibility to sudden cardiac death. Here we report the crystal structure of PSI in the context of the crystallized alphaVbeta3 ectodomain. The integrin PSI forms a two-stranded antiparallel beta-sheet flanked by two short helices; its long interstrand loop houses Pl(A) and may face the EGF2 domain. The integrin PSI contains four cysteine pairs connected in a 1-4, 2-8, 3-6, 5-7 pattern. An unexpected feature of the structure is that the final, eighth cysteine is located C-terminal to the Ig-like hybrid domain and is thus separated by the hybrid domain from the other seven cysteines of PSI. This architecture may be relevant to the evolution of integrins and should help refine the current models of integrin activation.

PubMed: 15299032
DOI: 10.1074/jbc.C400362200

Experimental method

X-RAY DIFFRACTION (3.1 Å)