1U8C
A novel adaptation of the integrin PSI domain revealed from its crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-02-25 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 130.000, 130.000, 307.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.2928 |
| Rwork | 0.293 |
| R-free | 0.36660 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.658 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 53673 | |
| Completeness [%] | 96.6 | 95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | PEG, MES, CA, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
