1TF6

CO-CRYSTAL STRUCTURE OF XENOPUS TFIIIA ZINC FINGER DOMAIN BOUND TO THE 5S RIBOSOMAL RNA GENE INTERNAL CONTROL REGION

Summary for 1TF6

• Entry DOI: 10.2210/pdb1tf6/pdb
• Descriptor: DNA (5'-D(*AP*CP*GP*GP*GP*CP*CP*TP*GP*GP*TP*TP*AP*GP*TP*AP*C P*CP*TP*GP*GP*AP* TP*GP*GP*GP*AP*GP*AP*CP*C)-3'), DNA (5'-D(*TP*GP*GP*TP*CP*TP*CP*CP*CP*AP*TP*CP*CP*AP*GP*GP*T P*AP*CP*TP*AP*AP* CP*CP*AP*GP*GP*CP*CP*CP*G)-3'), PROTEIN (TRANSCRIPTION FACTOR IIIA), ... (4 entities in total)
• Functional Keywords: complex (transcription regulation-dna), rna polymerase iii, transcription initiation, zinc finger protein, transcription-dna complex, transcription/dna
• Biological source: Xenopus laevis (African clawed frog)
• Cellular location: Nucleus: P03001
• Total number of polymer chains: 6
• Total formula weight: 83174.40

Authors

Nolte, R.T.,Conlin, R.M.,Harrison, S.C.,Brown, R.S. (deposition date: 1998-03-02, release date: 1998-07-10, Last modification date: 2024-02-14)

Primary citation

Nolte, R.T.,Conlin, R.M.,Harrison, S.C.,Brown, R.S.
Differing roles for zinc fingers in DNA recognition: structure of a six-finger transcription factor IIIA complex.
Proc.Natl.Acad.Sci.USA, 95:2938-2943, 1998

PubMed Abstract: The crystal structure of the six NH2-terminal zinc fingers of Xenopus laevis transcription factor IIIA (TFIIIA) bound with 31 bp of the 5S rRNA gene promoter has been determined at 3.1 A resolution. Individual zinc fingers are positioned differently in the major groove and across the minor groove of DNA to span the entire length of the duplex. These results show how TFIIIA can recognize several separated DNA sequences by using fewer fingers than necessary for continuous winding in the major groove.

PubMed: 9501194
DOI: 10.1073/pnas.95.6.2938

Experimental method

X-RAY DIFFRACTION (3.1 Å)