1TF6
CO-CRYSTAL STRUCTURE OF XENOPUS TFIIIA ZINC FINGER DOMAIN BOUND TO THE 5S RIBOSOMAL RNA GENE INTERNAL CONTROL REGION
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 113 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10 |
Detector | MARRESEARCH |
Spacegroup name | P 1 |
Unit cell lengths | 64.180, 64.712, 78.035 |
Unit cell angles | 90.07, 92.98, 102.95 |
Refinement procedure
Resolution | 8.000 - 3.100 |
R-factor | 0.308 |
Rwork | 0.308 |
R-free | 0.36300 |
Structure solution method | MIR |
RMSD bond length | 0.005 |
RMSD bond angle | 0.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CCP4 |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 3.000 |
Rmerge | 0.128 |
Total number of observations | 126746 * |
Number of reflections | 19034 |
Completeness [%] | 77.4 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | COMPLEX WAS CRYSTALLIZED FROM 22.5% PEG 4000, 165 MM NACL, 35 MM SODIUM ACETATE, 3.2 MM DTT, 9.2% (VOL/VOL) GLYCEROL, 1.8 MM NAN3, 1.8 MM CADAVERINE-2HCL, 5.5 MM TRIS-HCL, PH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.00K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | WATER | ||
10 | 1 | 2 | WATER | ||
11 | 1 | 2 | PEG 4000 | ||
2 | 1 | 1 | NACL | ||
3 | 1 | 1 | SODIUM ACETATE | ||
4 | 1 | 1 | DTT | ||
5 | 1 | 1 | GLYCEROL | ||
6 | 1 | 1 | NAN3 | ||
7 | 1 | 1 | CADAVERINE-2HCL | ||
8 | 1 | 1 | TRIS-HCL | ||
9 | 1 | 1 | PEG 4000 |