1STX

Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+

Summary for 1STX

• Entry DOI: 10.2210/pdb1stx/pdb
• Descriptor: 5'-D(*AP*AP*AP*GP*AP*T)-3', 5'-D(P*AP*TP*CP*TP*T)-3', Type II restriction enzyme EcoRV, ... (5 entities in total)
• Functional Keywords: restriction enzyme; ecorv; dna; x-ray crystallography, hydrolase-dna complex, hydrolase/dna
• Biological source: Escherichia coli
• Total number of polymer chains: 6
• Total formula weight: 63954.29

Authors

Horton, N.C.,Perona, J.J. (deposition date: 2004-03-25, release date: 2004-04-06, Last modification date: 2024-02-14)

• Primary citation: Horton, N.C.,Perona, J.J.; DNA cleavage by EcoRV endonuclease: two metal ions in three metal ion binding sites; Biochemistry, 43:6841-6857, 2004; PubMed: 15170321; DOI: 10.1021/bi0499056

Experimental method

X-RAY DIFFRACTION (2.1 Å)