1STX
Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (C, E) | 5'-D(*AP*AP*AP*GP*AP*T)-3' | polymer | 6 | 1841.3 | 2 | |||
| 2 | B, D (D, F) | 5'-D(P*AP*TP*CP*TP*T)-3' | polymer | 5 | 1470.0 | 2 | |||
| 3 | E, F (A, B) | Type II restriction enzyme EcoRV | polymer | 244 | 28501.1 | 2 | UniProt (P04390) Pfam (PF09233) | Escherichia coli | Endonuclease EcoRV; R.EcoRV; type II site-specific deoxyribonuclease EcoRV |
| 4 | G, H, I, J, K... (A, B) | MANGANESE (II) ION | non-polymer | 54.9 | 6 | Chemie (MN) | |||
| 5 | M, N, O, P, Q... (C, D, E, F, A...) | water | water | 18.0 | 383 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 245 (UniProt: P04390)
| PDB | External Database | Details |
|---|---|---|
| Ala 38 | Lys 37 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 63624.7 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 329.6 | |
| All* | Total formula weight | 63954.3 |
