1STX
Structure of the K38A mutant of EcoRV bound to cognate DNA and Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.710, 58.690, 82.100 |
Unit cell angles | 90.00, 107.70, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
R-factor | 0.2012 |
Rwork | 0.201 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 2.010 |
Phasing software | X-PLOR |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.100 |
Number of reflections | 32988 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 290 | 17% PEG 4000, 0.1M HEPES pH 7.5, 0.15M NaCl 50mM MnCl2, Vapor diffusion, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 4000 | ||
2 | 1 | 1 | HEPES | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | MnCl2 | ||
5 | 1 | 2 | PEG 4000 | ||
6 | 1 | 2 | HEPES | ||
7 | 1 | 2 | NaCl | ||
8 | 1 | 2 | MnCl2 |