1SKM

HhaI methyltransferase in complex with DNA containing an abasic south carbocyclic sugar at its target site

Summary for 1SKM

• Entry DOI: 10.2210/pdb1skm/pdb
• Related: 1FJX 1HMY 1MHT 9MHT
• Descriptor: 5'-D(*TP*CP*CP*AP*TP*GP*CP*GP*CP*TP*GP*AP*C)-3', 5'-D(*T*GP*TP*CP*AP*GP*(HCX)P*GP*CP*AP*TP*GP*G)-3', Modification methylase HhaI, ... (5 entities in total)
• Functional Keywords: protein-dna complex containing constrained abasic unnatural base, transferase-dna complex, transferase/dna
• Biological source: Haemophilus haemolyticus
• Total number of polymer chains: 3
• Total formula weight: 45246.74

Authors

Horton, J.R.,Ratner, G.,Banavali, N.,Huang, N.,Marquez, V.E.,MacKerell, A.D.,Cheng, X. (deposition date: 2004-03-05, release date: 2004-08-24, Last modification date: 2024-02-14)

• Primary citation: Horton, J.R.,Ratner, G.,Banavali, N.K.,Huang, N.,Choi, Y.,Maier, M.A.,Marquez, V.E.,MacKerell, A.D.,Cheng, X.; Caught in the act: visualization of an intermediate in the DNA base-flipping pathway induced by HhaI methyltransferase; Nucleic Acids Res., 32:3877-3886, 2004; PubMed: 15273274; DOI: 10.1093/nar/gkh701

Experimental method

X-RAY DIFFRACTION (2.2 Å)