1SKM
HhaI methyltransferase in complex with DNA containing an abasic south carbocyclic sugar at its target site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-10-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.1 |
Spacegroup name | H 3 2 |
Unit cell lengths | 95.681, 95.681, 315.683 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.530 - 2.200 |
R-factor | 0.183 |
Rwork | 0.183 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | CAD4 |
Data scaling software | SCALEPACK |
Phasing software | GLRF |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.530 | 2.250 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.179 | |
Number of reflections | 23477 | |
<I/σ(I)> | 18.4 | |
Completeness [%] | 81.9 | 36.9 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | PEG 6000, sodium cacodylate, magnesium acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 6000 | ||
2 | 1 | 1 | sodium cacodylate | ||
3 | 1 | 1 | magnesium acetate |