1RZ8

CRYSTAL STRUCTURE OF HUMAN ANTI-HIV-1 GP120-REACTIVE ANTIBODY 17B

Summary for 1RZ8

• Entry DOI: 10.2210/pdb1rz8/pdb
• Related: 1RZ7 1RZF 1RZG 1RZI 1RZJ 1RZK
• Descriptor: Fab 17b light chain, Fab 17b heavy chain (3 entities in total)
• Functional Keywords: hiv-1; gp120; cd4i; antibodies; tyrosine sulfation; vh-gene usage, immune system
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 4
• Total formula weight: 95456.21

Authors

Huang, C.C.,Venturi, M.,Majeed, S.,Moore, M.J.,Phogat, S.,Zhang, M.-Y.,Dimitrov, D.S.,Hendrickson, W.A.,Robinson, J.,Sodroski, J.,Wyatt, R.,Choe, H.,Farzan, M.,Kwong, P.D. (deposition date: 2003-12-24, release date: 2004-02-03, Last modification date: 2024-10-16)

Primary citation

Huang, C.C.,Venturi, M.,Majeed, S.,Moore, M.J.,Phogat, S.,Zhang, M.-Y.,Dimitrov, D.S.,Hendrickson, W.A.,Robinson, J.,Sodroski, J.,Wyatt, R.,Choe, H.,Farzan, M.,Kwong, P.D.
Structural basis of tyrosine sulfation and VH-gene usage in antibodies that recognize the HIV type 1 coreceptor-binding site on gp120
Proc.Natl.Acad.Sci.USA, 101:2706-2711, 2004

PubMed Abstract: The conserved surface of the HIV-1 gp120 envelope glycoprotein that binds to the HIV-1 coreceptor is protected from humoral recognition by multiple layers of camouflage. Here we present sequence and genomic analyses for 12 antibodies that pierce these defenses and determine the crystal structures of 5. The data reveal mechanisms and atomic-level details for three unusual immune features: posttranslational mimicry of coreceptor by tyrosine sulfation of antibody, an alternative molecular mechanism controlling such sulfation, and highly selective V(H)-gene usage. When confronted by extraordinary viral defenses, the immune system unveils novel adaptive capabilities, with tyrosine sulfation enhancing the vocabulary of antigen recognition.

PubMed: 14981267
DOI: 10.1073/pnas.0308527100

Experimental method

X-RAY DIFFRACTION (2.3 Å)