1RRV

X-ray crystal structure of TDP-vancosaminyltransferase GtfD as a complex with TDP and the natural substrate, desvancosaminyl vancomycin.

Summary for 1RRV

• Entry DOI: 10.2210/pdb1rrv/pdb
• Related: 1AA5 1C0Q 1C0R 1FVM 1GAC 1GHG 1PN3 1PNV 1QD8 1SHO
• Related PRD ID: PRD_000205
• Descriptor: GLYCOSYLTRANSFERASE GTFD, DESVANCOSAMINYL VANCOMYCIN, POTASSIUM ION, ... (7 entities in total)
• Functional Keywords: gt-b, glycosyltransferase, rossmann fold, glycopeptide, vacosamycin, antibiotic, transferase-antibiotic complex, transferase/antibiotic
• Biological source: AMYCOLATOPSIS ORIENTALIS; More
• Total number of polymer chains: 4
• Total formula weight: 91630.88

Authors

Mulichak, A.M.,Lu, W.,Losey, H.C.,Walsh, C.T.,Garavito, R.M. (deposition date: 2003-12-09, release date: 2004-05-18, Last modification date: 2023-11-15)

Primary citation

Mulichak, A.M.,Lu, W.,Losey, H.C.,Walsh, C.T.,Garavito, R.M.
Crystal Structure of Vancosaminyltransferase Gtfd from the Vancomycin Biosynthetic Pathway: Interactions with Acceptor and Nucleotide Ligands
Biochemistry, 43:5170-, 2004

PubMed Abstract: The TDP-vancosaminyltransferase GtfD catalyzes the attachment of L-vancosamine to a monoglucosylated heptapeptide intermediate during the final stage of vancomycin biosynthesis. Glycosyltransferases from this and similar antibiotic pathways are potential tools for the design of new compounds that are effective against vancomycin resistant bacterial strains. We have determined the X-ray crystal structure of GtfD as a complex with TDP and the natural glycopeptide substrate at 2.0 A resolution. GtfD, a member of the bidomain GT-B glycosyltransferase superfamily, binds TDP in the interdomain cleft, while the aglycone acceptor binds in a deep crevice in the N-terminal domain. However, the two domains are more interdependent in terms of substrate binding and overall structure than was evident in the structures of closely related glycosyltransferases GtfA and GtfB. Structural and kinetic analyses support the identification of Asp13 as a catalytic general base, with a possible secondary role for Thr10. Several residues have also been identified as being involved in donor sugar binding and recognition.

PubMed: 15122882
DOI: 10.1021/BI036130C

Experimental method

X-RAY DIFFRACTION (2 Å)