1RCJ

Crystal structure of E166A mutant of SHV-1 beta-lactamase with the trans-enamine intermediate of tazobactam

Summary for 1RCJ

Entry DOI	10.2210/pdb1rcj/pdb
Related	1G56 1n9b
Descriptor	Beta-lactamase SHV-1, CYCLOHEXYL-HEXYL-BETA-D-MALTOSIDE, TAZOBACTAM TRANS-ENAMINE INTERMEDIATE, ... (4 entities in total)
Functional Keywords	beta-lactamase, beta-lactam hydrolase, penicillinase, detergent binding, inhibitor design, covalent intermediate, hydrolase
Biological source	Klebsiella pneumoniae
Total number of polymer chains	1
Total formula weight	30168.49
Authors	Padayatti, P.S.,Helfand, M.S.,Totir, M.A.,Carey, M.P.,Hujer, A.M.,Carey, P.R.,Bonomo, R.A.,van den Akker, F. (deposition date: 2003-11-04, release date: 2004-02-17, Last modification date: 2023-08-23)
Primary citation	Padayatti, P.S.,Helfand, M.S.,Totir, M.A.,Carey, M.P.,Hujer, A.M.,Carey, P.R.,Bonomo, R.A.,Van Den Akker, F. Tazobactam Forms a Stoichiometric trans-Enamine Intermediate in the E166A Variant of SHV-1 beta-Lactamase: 1.63 A Crystal Structure Biochemistry, 43:843-848, 2004 Cited by PubMed: 14744126 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.63 Å)

Structure validation