Bacterial cytosine deaminase D314A mutant bound to 5-fluoro-4-(S)-hydroxyl-3,4-dihydropyrimidine.

Summary for 1RA5

Related1K7O 1R9X 1R9Y 1R9Z 1RA0 1RAK 1RB7
DescriptorCytosine deaminase, FE (III) ION, (4S)-5-FLUORO-4-HYDROXY-3,4-DIHYDROPYRIMIDIN-2(1H)-ONE, ... (5 entities in total)
Functional Keywordscytosine deaminase, alpha-beta barrel, hexamer, conformational change, d314a mutant, hydrolase
Biological sourceEscherichia coli
Total number of polymer chains1
Total molecular weight48096.02
Mahan, S.D.,Ireton, G.C.,Stoddard, B.L.,Black, M.E. (deposition date: 2003-10-31, release date: 2004-10-05, Last modification date: 2011-07-13)
Primary citation
Mahan, S.D.,Ireton, G.C.,Knoeber, C.,Stoddard, B.L.,Black, M.E.
Random mutagenesis and selection of Escherichia coli cytosine deaminase for cancer gene therapy.
Protein Eng.Des.Sel., 17:625-633, 2004
PubMed: 15381761 (PDB entries with the same primary citation)
DOI: 10.1093/protein/gzh074
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.190140 1.4% 10.4%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
PDB entries from 2020-12-02