1PGR

2:2 COMPLEX OF G-CSF WITH ITS RECEPTOR

Summary for 1PGR

• Entry DOI: 10.2210/pdb1pgr/pdb
• Descriptor: PROTEIN (GRANULOCYTE COLONY-STIMULATING FACTOR), PROTEIN (G-CSF RECEPTOR) (2 entities in total)
• Functional Keywords: class1 cytokine, hematopoietic receptor, signal transduction, cytokine
• Biological source: Homo sapiens (human); More
• Cellular location: Secreted: P09919; Membrane; Single-pass type I membrane protein: P40223
• Total number of polymer chains: 8
• Total formula weight: 173108.10

Authors

Aritomi, M.,Kunishima, N.,Okamoto, T.,Kuroki, R.,Ota, Y.,Morikawa, K. (deposition date: 1999-03-08, release date: 2000-03-08, Last modification date: 2024-11-13)

Primary citation

Aritomi, M.,Kunishima, N.,Okamoto, T.,Kuroki, R.,Ota, Y.,Morikawa, K.
Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme.
Nature, 401:713-717, 1999

PubMed Abstract: Granulocyte colony-stimulating factor (GCSF) is the principal growth factor regulating the maturation, proliferation and differentiation of the precursor cells of neutrophilic granulocytes and is used to treat neutropenia. GCSF is a member of the long-chain subtype of the class 1 cytokine superfamily, which includes growth hormone, erythropoietin, interleukin 6 and oncostatin M. Here we have determined the crystal structure of GCSF complexed to the BN-BC domains, the principal ligand-binding region of the GCSF receptor (GCSFR). The two receptor domains form a complex in a 2:2 ratio with the ligand, with a non-crystallographic pseudo-twofold axis through primarily the interdomain region and secondarily the BC domain. This structural view of a gp130-type receptor-ligand complex presents a new molecular basis for cytokine-receptor recognition.

PubMed: 10537111
DOI: 10.1038/44394

Experimental method

X-RAY DIFFRACTION (3.5 Å)