1PGR
2:2 COMPLEX OF G-CSF WITH ITS RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-12 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 125.725, 125.725, 373.305 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.500 |
| Rwork | 0.317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1igr |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.500 |
| Rmerge | 0.198 |
| Total number of observations | 173168 * |
| Number of reflections | 26690 * |
| Completeness [%] | 69.1 |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | manuscript in preparation * |
