1NCE

Crystal structure of a ternary complex of E. coli thymidylate synthase D169C with dUMP and the antifolate CB3717

Summary for 1NCE

• Entry DOI: 10.2210/pdb1nce/pdb
• Related: 1bjg 1dna 1kce
• Descriptor: Thymidylate synthase, 2'-DEOXYURIDINE 5'-MONOPHOSPHATE, 10-PROPARGYL-5,8-DIDEAZAFOLIC ACID, ... (4 entities in total)
• Functional Keywords: beta-sheet interface, protein-dump-cofactor analog complex, asymmetric dimer, biosynthetic protein
• Biological source: Escherichia coli, Escherichia coli O157:H7 (,)
• Cellular location: Cytoplasm : P0A884
• Total number of polymer chains: 2
• Total formula weight: 62666.74

Authors

Birdsall, D.L.,Finer-Moore, J.,Stroud, R.M. (deposition date: 2002-12-05, release date: 2002-12-25, Last modification date: 2023-08-16)

• Primary citation: Birdsall, D.L.,Finer-Moore, J.,Stroud, R.M.; The only active mutant of thymidylate synthase D169, a residue far from the site of methyl transfer, demonstrates the exquisite nature of enzyme specificity.; Protein Eng., 16:229-240, 2003; PubMed: 12702803; DOI: 10.1093/proeng/gzg020

Experimental method

X-RAY DIFFRACTION (2.4 Å)