1N9S

Crystal structure of yeast SmF in spacegroup P43212

1N9S の概要

• エントリーDOI: 10.2210/pdb1n9s/pdb
• 関連するPDBエントリー: 1I4K 1I5L 1I81 1I8F 1JR1 1N9R
• 分子名称: Small nuclear ribonucleoprotein F (1 entity in total)
• 機能のキーワード: snrnp, sm protein, heptamer, translation
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus: P54999
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 148595.40

構造登録者

Collins, B.M.,Cubeddu, L.,Naidoo, N.,Harrop, S.J.,Kornfeld, G.D.,Dawes, I.W.,Curmi, P.M.G.,Mabbutt, B.C. (登録日: 2002-11-26, 公開日: 2002-12-13, 最終更新日: 2023-08-16)

主引用文献

Collins, B.M.,Cubeddu, L.,Naidoo, N.,Harrop, S.J.,Kornfeld, G.D.,Dawes, I.W.,Curmi, P.M.G.,Mabbutt, B.C.
Homomeric ring assemblies of eukaryotic Sm proteins have affinity for both RNA and DNA: Crystal structure of an oligomeric complex of yeast SmF
J.Biol.Chem., 278:17291-17298, 2003

PubMed Abstract: Sm and Sm-like proteins are key components of small ribonucleoproteins involved in many RNA and DNA processing pathways. In eukaryotes, these complexes contain seven unique Sm or Sm-like (Lsm) proteins assembled as hetero-heptameric rings, whereas in Archaea and bacteria six or seven-membered rings are made from only a single polypeptide chain. Here we show that single Sm and Lsm proteins from yeast also have the capacity to assemble into homo-oligomeric rings. Formation of homo-oligomers by the spliceosomal small nuclear ribonucleoprotein components SmE and SmF preclude hetero-interactions vital to formation of functional small nuclear RNP complexes in vivo. To better understand these unusual complexes, we have determined the crystal structure of the homomeric assembly of the spliceosomal protein SmF. Like its archaeal/bacterial homologs, the SmF complex forms a homomeric ring but in an entirely novel arrangement whereby two heptameric rings form a co-axially stacked dimer via interactions mediated by the variable loops of the individual SmF protein chains. Furthermore, we demonstrate that the homomeric assemblies of yeast Sm and Lsm proteins are capable of binding not only to oligo(U) RNA but, in the case of SmF, also to oligo(dT) single-stranded DNA.

PubMed: 12618433
DOI: 10.1074/jbc.M211826200

実験手法

X-RAY DIFFRACTION (3.5 Å)