1N9S
Crystal structure of yeast SmF in spacegroup P43212
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Wavelength(s) | 1.000 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 105.635, 105.635, 235.563 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.500 |
R-factor | 0.29193 |
Rwork | 0.292 |
R-free | 0.29700 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID: 1N9R |
RMSD bond length | 0.023 |
RMSD bond angle | 2.300 * |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.000 | 3.690 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.064 | 0.353 * |
Number of reflections | 16292 | |
<I/σ(I)> | 23.5 | 6 |
Completeness [%] | 99.3 | 99.3 |
Redundancy | 6.7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | Tris, PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Tris-NaCl | ||
2 | 1 | drop | protein | 20 (mg/ml) | |
3 | 1 | reservoir | PEG3350 | 24 (%) | |
4 | 1 | reservoir | Tris | 0.1 (M) | pH8.5 |
5 | 1 | reservoir | sodium acetate | 0.1 (M) |