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1N9S

Crystal structure of yeast SmF in spacegroup P43212

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-2
Synchrotron siteSSRL
BeamlineBL9-2
Temperature [K]100
Detector technologyAREA DETECTOR
Wavelength(s)1.000
Spacegroup nameP 43 21 2
Unit cell lengths105.635, 105.635, 235.563
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 3.500
R-factor0.29193
Rwork0.292
R-free0.29700

*

Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)PDB ID: 1N9R
RMSD bond length0.023
RMSD bond angle2.300

*

Data reduction softwareMOSFLM
Data scaling softwareSCALA
Phasing softwareAMoRE
Refinement softwareREFMAC (5.0)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]95.0003.690
High resolution limit [Å]3.5003.500
Rmerge0.0640.353

*

Number of reflections16292
<I/σ(I)>23.56
Completeness [%]99.399.3
Redundancy6.76.8
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP8.5289Tris, PEG 3350, sodium acetate, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 289K
Crystallization Reagents in Literatures
IDcrystal IDsolutionreagent nameconcentration (unit)details
11dropTris-NaCl
21dropprotein20 (mg/ml)
31reservoirPEG335024 (%)
41reservoirTris0.1 (M)pH8.5
51reservoirsodium acetate0.1 (M)

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PDB entries from 2024-11-06

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