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1I4K

CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS AT 2.5A RESOLUTION

Summary for 1I4K
Entry DOI10.2210/pdb1i4k/pdb
Related1B34 1D3B
DescriptorPUTATIVE SNRNP SM-LIKE PROTEIN, CITRIC ACID (3 entities in total)
Functional Keywordssnrnp, sm, core snrnp domain, rna binding protein
Biological sourceArchaeoglobus fulgidus
Total number of polymer chains28
Total formula weight235352.10
Authors
Toro, I.,Thore, S.,Mayer, C.,Basquin, J.,Seraphin, B.,Suck, D. (deposition date: 2001-02-22, release date: 2001-08-22, Last modification date: 2024-04-03)
Primary citationToro, I.,Thore, S.,Mayer, C.,Basquin, J.,Seraphin, B.,Suck, D.
RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex.
EMBO J., 20:2293-2303, 2001
Cited by
PubMed Abstract: Eukaryotic Sm and Sm-like proteins associate with RNA to form the core domain of ribonucleoprotein particles involved in pre-mRNA splicing and other processes. Recently, putative Sm proteins of unknown function have been identified in Archaea. We show by immunoprecipitation experiments that the two Sm proteins present in Archaeoglobus fulgidus (AF-Sm1 and AF-Sm2) associate with RNase P RNA in vivo, suggesting a role in tRNA processing. The AF-Sm1 protein also interacts specifically with oligouridylate in vitro. We have solved the crystal structures of this protein and a complex with RNA. AF-Sm1 forms a seven-membered ring, with the RNA interacting inside the central cavity on one face of the doughnut-shaped complex. The bases are bound via stacking and specific hydrogen bonding contacts in pockets lined by residues highly conserved in archaeal and eukaryotic Sm proteins, while the phosphates remain solvent accessible. A comparison with the structures of human Sm protein dimers reveals closely related monomer folds and intersubunit contacts, indicating that the architecture of the Sm core domain and RNA binding have been conserved during evolution.
PubMed: 11331594
DOI: 10.1093/emboj/20.9.2293
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.5 Å)
Structure validation

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