1MQ8

Crystal structure of alphaL I domain in complex with ICAM-1

1MQ8 の概要

• エントリーDOI: 10.2210/pdb1mq8/pdb
• 関連するPDBエントリー: 1MQ9 1MQA
• 分子名称: intercellular adhesion molecule-1, Integrin alpha-L, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: ig superfamily, rossmann fold, metal mediated protein interface, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 106260.94

構造登録者

Shimaoka, M.,Xiao, T.,Liu, J.-H.,Yang, Y.,Dong, Y.,Jun, C.-D.,McCormack, A.,Zhang, R.,Joachimiak, A.,Takagi, J.,Wang, J.-H.,Springer, T.A. (登録日: 2002-09-15, 公開日: 2003-01-14, 最終更新日: 2024-11-13)

主引用文献

Shimaoka, M.,Xiao, T.,Liu, J.-H.,Yang, Y.,Dong, Y.,Jun, C.-D.,McCormack, A.,Zhang, R.,Joachimiak, A.,Takagi, J.,Wang, J.-H.,Springer, T.A.
Structures of the aL I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation
Cell(Cambridge,Mass.), 112:99-111, 2003

PubMed Abstract: The structure of the I domain of integrin alpha L beta 2 bound to the Ig superfamily ligand ICAM-1 reveals the open ligand binding conformation and the first example of an integrin-IgSF interface. The I domain Mg2+ directly coordinates Glu-34 of ICAM-1, and a dramatic swing of I domain residue Glu-241 enables a critical salt bridge. Liganded and unliganded structures for both high- and intermediate-affinity mutant I domains reveal that ligand binding can induce conformational change in the alpha L I domain and that allosteric signals can convert the closed conformation to intermediate or open conformations without ligand binding. Pulling down on the C-terminal alpha 7 helix with introduced disulfide bonds ratchets the beta 6-alpha 7 loop into three different positions in the closed, intermediate, and open conformations, with a progressive increase in affinity.

PubMed: 12526797
DOI: 10.1016/S0092-8674(02)01257-6

実験手法

X-RAY DIFFRACTION (3.3 Å)