1MQ8
Crystal structure of alphaL I domain in complex with ICAM-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-01-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9100 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.560, 62.868, 81.517 |
| Unit cell angles | 95.39, 106.67, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.28 |
| Rwork | 0.264 |
| R-free | 0.31300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ic1 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.700 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.094 | 0.412 |
| Total number of observations | 23330 * | |
| Number of reflections | 12338 * | |
| <I/σ(I)> | 7.7 | 1.5 |
| Completeness [%] | 92.3 | 70.7 |
| Redundancy | 1.9 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 25% PEG 4000, 0.1 M sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP at 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-20 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 25 (%) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
