1M04

Mutant Streptomyces plicatus beta-hexosaminidase (D313N) in complex with product (GlcNAc)

Summary for 1M04

Related1M01 1M03 1hp5 1jak
DescriptorBeta-N-acetylhexosaminidase, N-ACETYL-D-GLUCOSAMINE, CHLORIDE ION, ... (6 entities in total)
Functional Keywordssubstrate assisted catalysis, hexosaminidase, family 20 glycosidase, hydrolase
Biological sourceStreptomyces plicatus
Total number of polymer chains1
Total molecular weight56641.52
Authors
Williams, S.J.,Mark, B.L.,Vocadlo, D.J.,James, M.N.G.,Withers, S.G. (deposition date: 2002-06-11, release date: 2002-12-11, Last modification date: 2011-07-13)
Primary citation
Williams, S.J.,Mark, B.L.,Vocadlo, D.J.,James, M.N.,Withers, S.G.
Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state.
J.Biol.Chem., 277:40055-40065, 2002
PubMed: 12171933 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M206481200
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.95 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.211501.0%2.0%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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More Biological unit images

Molmil generated image of 1m04
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Molmil generated image of 1m04
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Molmil generated image of 1m04
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(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (1m04.pdb1.gz [85.73 KB])
Coordinate files for Biological unit (1m04.pdb2.gz [168.17 KB])