1M04
Mutant Streptomyces plicatus beta-hexosaminidase (D313N) in complex with product (GlcNAc)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Beta-N-acetylhexosaminidase | polymer | 512 | 56125.8 | 1 | UniProt (O85361) Pfam (PF02838) Pfam (PF00728) | Streptomyces plicatus | beta-hexosaminidase, SpHEX |
| 2 | B (A) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 3 | C, D, E (A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 4 | F (A) | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
| 5 | G (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 6 | H (A) | water | water | 18.0 | 287 | Chemie (HOH) |
Sequence modifications
A: 3 - 506 (UniProt: O85361)
| PDB | External Database | Details |
|---|---|---|
| Met -5 | - | cloning artifact |
| His -4 | - | expression tag |
| His -3 | - | expression tag |
| His -2 | - | expression tag |
| His -1 | - | expression tag |
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| His 2 | - | expression tag |
| Asn 313 | Asp 313 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 56125.8 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 515.7 | |
| All* | Total formula weight | 56641.5 |
