1M03

Mutant Streptomyces plicatus beta-hexosaminidase (D313A) in complex with product (GlcNAc)

Summary for 1M03

Related1M01 1M04 1hp5 1JAK
DescriptorBeta-N-acetylhexosaminidase, N-ACETYL-D-GLUCOSAMINE, CHLORIDE ION, ... (6 entities in total)
Functional Keywordssubstrate assisted catalysis, family 20 glycosidase, beta-hexosaminidase, hydrolase
Biological sourceStreptomyces plicatus
Total number of polymer chains1
Total molecular weight56690.59
Authors
Williams, S.J.,Mark, B.L.,Vocadlo, D.J.,James, M.N.G.,Withers, S.G. (deposition date: 2002-06-11, release date: 2002-12-11, Last modification date: 2011-11-16)
Primary citation
Williams, S.J.,Mark, B.L.,Vocadlo, D.J.,James, M.N.,Withers, S.G.
Aspartate 313 in the Streptomyces plicatus hexosaminidase plays a critical role in substrate-assisted catalysis by orienting the 2-acetamido group and stabilizing the transition state.
J.Biol.Chem., 277:40055-40065, 2002
PubMed: 12171933 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M206481200
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.9 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers0.205500.8%1.2%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution

More Asymmetric unit images

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More Biological unit images

Molmil generated image of 1m03
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Molmil generated image of 1m03
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Molmil generated image of 1m03
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(*)In the case of coarse surface representation, the asymmetric unit is shown as red ribbon representation.
Coordinate files for Biological unit (1m03.pdb1.gz [85.86 KB])
Coordinate files for Biological unit (1m03.pdb2.gz [168.64 KB])