1M03

Mutant Streptomyces plicatus beta-hexosaminidase (D313A) in complex with product (GlcNAc)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Beta-N-acetylhexosaminidase	polymer	512	56082.8	1	UniProt (O85361) Pfam (PF02838) Pfam (PF00728) In PDB	Streptomyces plicatus	beta-hexosaminidase, SpHEX
2	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
3	A	CHLORIDE ION	non-polymer		35.5	3	Chemie (CL)
4	A	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	A	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
6		water	water		18.0	295	Chemie (HOH)

Sequence modifications

A: 3 - 506 (UniProt: O85361)

PDB	External Database	Details
Met -5	-	cloning artifact
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
His 1	-	expression tag
His 2	-	expression tag
Ala 313	Asp 313	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	56082.8
Non-Polymers*	Number of molecules	7
	Total formula weight	607.8
All*	Total formula weight	56690.6

*Water molecules are not included.