1M03
Mutant Streptomyces plicatus beta-hexosaminidase (D313A) in complex with product (GlcNAc)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Beta-N-acetylhexosaminidase | polymer | 512 | 56082.8 | 1 | UniProt (O85361) Pfam (PF02838) Pfam (PF00728) In PDB | Streptomyces plicatus | beta-hexosaminidase, SpHEX |
2 | A | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
3 | A | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
4 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
5 | A | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
6 | water | water | 18.0 | 295 | Chemie (HOH) |
Sequence modifications
A: 3 - 506 (UniProt: O85361)
PDB | External Database | Details |
---|---|---|
Met -5 | - | cloning artifact |
His -4 | - | expression tag |
His -3 | - | expression tag |
His -2 | - | expression tag |
His -1 | - | expression tag |
His 0 | - | expression tag |
His 1 | - | expression tag |
His 2 | - | expression tag |
Ala 313 | Asp 313 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 1 |
Total formula weight | 56082.8 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 607.8 | |
All* | Total formula weight | 56690.6 |