1L1V

UNUSUAL ACTD/DNA_TA COMPLEX STRUCTURE

1L1V の概要

• エントリーDOI: 10.2210/pdb1l1v/pdb
• 関連するPDBエントリー: 173D 1A7Y 1A7Z 1DSC 1DSD 1FJA 1I3W 1MNV 1OVF 1QFI 1UNJ 1UNM 209D 2D55 316D
• 関連するBIRD辞書のPRD_ID: PRD_000001
• 分子名称: 5'-D(*GP*TP*CP*AP*CP*CP*GP*AP*C)-3', ACTINOMYCIN D (2 entities in total)
• 機能のキーワード: actinomycin d, actinomycin, antibiotic, chromophore, anti cancer, antitumor, depsipeptide, mismatch, dna-antibiotic complex, dna/antibiotic
• 由来する生物種: STREPTOMYCES ANTIBIOTICUS
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 3992.23

構造登録者

Chou, S.-H.,Chin, K.-H.,Chen, F.-M. (登録日: 2002-02-20, 公開日: 2002-03-06, 最終更新日: 2024-11-13)

主引用文献

Chou, S.H.,Chin, K.H.,Chen, F.M.
Looped Out and Perpendicular: Deformation of Watson-Crick Base Pair Associated with Actinomycin D Binding.
Proc.Natl.Acad.Sci.USA, 99:6625-, 2002

PubMed Abstract: Many anticancer drugs interact directly with DNA to exert their biological functions. To date, all noncovalent, intercalating drugs interact with DNA exclusively by inserting their chromophores into base steps to form elongated and unwound duplex structures without disrupting the flanking base pairs. By using actinomycin D (ActD)-5'-GXC/CYG-5' complexes as examples, we have found a rather unusual interaction mode for the intercalated drug; the central Watson-Crick X/Y base pairs are looped out and displaced by the ActD chromophore. The looped-out bases are not disordered but interact perpendicularly with the base/chromophore and form specific H bonds with DNA. Such a complex structure provides intriguing insights into how ligand interacts with DNA and enlarges the repertoires for sequence-specific DNA recognition.

PubMed: 12011426
DOI: 10.1073/PNAS.102580399

実験手法

SOLUTION NMR