1J2J

Crystal structure of GGA1 GAT N-terminal region in complex with ARF1 GTP form

Summary for 1J2J

• Entry DOI: 10.2210/pdb1j2j/pdb
• Related: 1J2H 1J2I
• Descriptor: ADP-ribosylation factor 1, ADP-ribosylation factor binding protein GGA1, MAGNESIUM ION, ... (6 entities in total)
• Functional Keywords: protein transport
• Biological source: Mus musculus (house mouse); More
• Cellular location: Golgi apparatus: Q8BSL7; Golgi apparatus, trans-Golgi network membrane; Peripheral membrane protein: Q9UJY5
• Total number of polymer chains: 2
• Total formula weight: 25121.14

Authors

Shiba, T.,Kawasaki, M.,Takatsu, H.,Nogi, T.,Matsugaki, N.,Igarashi, N.,Suzuki, M.,Kato, R.,Nakayama, K.,Wakatsuki, S. (deposition date: 2003-01-05, release date: 2003-05-06, Last modification date: 2023-10-25)

Primary citation

Shiba, T.,Kawasaki, M.,Takatsu, H.,Nogi, T.,Matsugaki, N.,Igarashi, N.,Suzuki, M.,Kato, R.,Nakayama, K.,Wakatsuki, S.
Molecular mechanism of membrane recruitment of GGA by ARF in lysosomal protein transport
NAT.STRUCT.BIOL., 10:386-393, 2003

PubMed Abstract: GGAs are critical for trafficking soluble proteins from the trans-Golgi network (TGN) to endosomes/lysosomes through interactions with TGN-sorting receptors, ADP-ribosylation factor (ARF) and clathrin. ARF-GTP bound to TGN membranes recruits its effector GGA by binding to the GAT domain, thus facilitating recognition of GGA for cargo-loaded receptors. Here we report the X-ray crystal structures of the human GGA1-GAT domain and the complex between ARF1-GTP and the N-terminal region of the GAT domain. When unbound, the GAT domain forms an elongated bundle of three a-helices with a hydrophobic core. Structurally, this domain, combined with the preceding VHS domain, resembles CALM, an AP180 homolog involved in endocytosis. In the complex with ARF1-GTP, a helix-loop-helix of the N-terminal part of GGA1-GAT interacts with the switches 1 and 2 of ARF1 predominantly in a hydrophobic manner. These data reveal a molecular mechanism underlying membrane recruitment of adaptor proteins by ARF-GTP.

PubMed: 12679809
DOI: 10.1038/nsb920

Experimental method

X-RAY DIFFRACTION (1.6 Å)