1J2J

Crystal structure of GGA1 GAT N-terminal region in complex with ARF1 GTP form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-14
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	49.410, 61.894, 76.874
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.600
R-factor	0.19988
Rwork	0.198
R-free	0.22700 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1J2I
RMSD bond length	0.012
RMSD bond angle	1.560 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.048	0.245
Total number of observations	194166 *
Number of reflections	30626 *
<I/σ(I)>	11	3
Completeness [%]	96.4	83.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	293	PEG3350, KI, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	10 (%)
2	1	reservoir		0.2 (M)
3	1	drop		5 (mM)
4	1	drop	Tris-HCl	10 (mM)	pH8.0
5	1	drop	protein	30 (mg/ml)