1HZH

CRYSTAL STRUCTURE OF THE INTACT HUMAN IGG B12 WITH BROAD AND POTENT ACTIVITY AGAINST PRIMARY HIV-1 ISOLATES: A TEMPLATE FOR HIV VACCINE DESIGN

1HZH の概要

• エントリーDOI: 10.2210/pdb1hzh/pdb
• 分子名称: IMMUNOGLOBULIN HEAVY CHAIN, IMMUNOGLOBULIN LIGHT CHAIN,Uncharacterized protein, beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[beta-D-galactopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: immunoglobulin, antibody, b12, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Secreted : P0DOX5
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 151628.99

構造登録者

Saphire, E.O.,Burton, D.R.,Wilson, I.A. (登録日: 2001-01-24, 公開日: 2001-08-15, 最終更新日: 2024-11-20)

主引用文献

Saphire, E.O.,Parren, P.W.,Pantophlet, R.,Zwick, M.B.,Morris, G.M.,Rudd, P.M.,Dwek, R.A.,Stanfield, R.L.,Burton, D.R.,Wilson, I.A.
Crystal structure of a neutralizing human IGG against HIV-1: a template for vaccine design.
Science, 293:1155-1159, 2001

PubMed Abstract: We present the crystal structure at 2.7 angstrom resolution of the human antibody IgG1 b12. Antibody b12 recognizes the CD4-binding site of human immunodeficiency virus-1 (HIV-1) gp120 and is one of only two known antibodies against gp120 capable of broad and potent neutralization of primary HIV-1 isolates. A key feature of the antibody-combining site is the protruding, finger-like long CDR H3 that can penetrate the recessed CD4-binding site of gp120. A docking model of b12 and gp120 reveals severe structural constraints that explain the extraordinary challenge in eliciting effective neutralizing antibodies similar to b12. The structure, together with mutagenesis studies, provides a rationale for the extensive cross-reactivity of b12 and a valuable framework for the design of HIV-1 vaccines capable of eliciting b12-like activity.

PubMed: 11498595
DOI: 10.1126/science.1061692

実験手法

X-RAY DIFFRACTION (2.7 Å)