1HPT

THREE-DIMENSIONAL STRUCTURE OF A RECOMBINANT VARIANT OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL TYPE)

Summary for 1HPT

• Entry DOI: 10.2210/pdb1hpt/pdb
• Descriptor: PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL TYPE) VARIANT 3 (2 entities in total)
• Functional Keywords: serine protease inhibitor
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: P00995
• Total number of polymer chains: 1
• Total formula weight: 6347.09

Authors

Hecht, H.J.,Szardenings, M.,Collins, J.,Schomburg, D. (deposition date: 1992-03-27, release date: 1993-10-31, Last modification date: 2024-10-16)

Primary citation

Hecht, H.J.,Szardenings, M.,Collins, J.,Schomburg, D.
Three-dimensional structure of a recombinant variant of human pancreatic secretory trypsin inhibitor (Kazal type).
J.Mol.Biol., 225:1095-1103, 1992

PubMed Abstract: A modified version of the human pancreatic trypsin inhibitor (PSTI), generated in a protein-design project, has been crystallized in spacegroup P4(3) with lattice constants a = 40.15 A, c = 33.91 A. The structure has been solved by molecular replacement. Refinement of the structure by simulated annealing and conventional restrained least-squares yielded for 8.0 to 2.3 A data a final R-value of 19.1%. Differences to the known structures of porcine PSTI complexed with trypsinogen and modified human PSTI complexed with chymotrypsinogen occur at the flexible N-terminal part of the molecule. These differences are influenced by crystal packing, as are low temperature factors for the binding loop. The geometry of the binding loop is similar to the complexed structures.

PubMed: 1613792
DOI: 10.1016/0022-2836(92)90107-U

Experimental method

X-RAY DIFFRACTION (2.3 Å)