1HPT

THREE-DIMENSIONAL STRUCTURE OF A RECOMBINANT VARIANT OF HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR (KAZAL TYPE)

Experimental procedure

Spacegroup name	P 43
Unit cell lengths	40.150, 40.150, 33.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
R-factor	0.191
Rwork	0.191
RMSD bond length	0.011
RMSD bond angle	2.700
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	2.460 *
High resolution limit [Å]	2.300 *
Rmerge	0.069 *
Total number of observations	2200 *
Number of reflections	4612 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	8.3 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	2	ammonium sulfate	2.0 (M)
2	1	2	Tris-HCl	100 (mM)
3	1	1	inhibitor	10 (mg/ml)