1HCQ

THE CRYSTAL STRUCTURE OF THE ESTROGEN RECEPTOR DNA-BINDING DOMAIN BOUND TO DNA: HOW RECEPTORS DISCRIMINATE BETWEEN THEIR RESPONSE ELEMENTS

Summary for 1HCQ

• Entry DOI: 10.2210/pdb1hcq/pdb
• Descriptor: DNA (5'-D(*CP*CP*AP*GP*GP*TP*CP*AP*CP*AP*GP*TP*GP*AP*CP*CP*T P*G)-3'), DNA (5'-D(*CP*CP*AP*GP*GP*TP*CP*AP*CP*TP*GP*TP*GP*AP*CP*CP*T P*G)-3'), PROTEIN (ESTROGEN RECEPTOR), ... (5 entities in total)
• Functional Keywords: protein-dna complex, complexed with drug, transcription-dna complex, transcription/dna
• Biological source: Homo sapiens (human)
• Cellular location: Isoform 1: Nucleus. Isoform 3: Nucleus: P03372
• Total number of polymer chains: 8
• Total formula weight: 61356.59

Authors

Schwabe, J.W.R.,Chapman, L.,Finch, J.T.,Rhodes, D. (deposition date: 1995-01-04, release date: 1995-11-23, Last modification date: 2024-11-06)

Primary citation

Schwabe, J.W.,Chapman, L.,Finch, J.T.,Rhodes, D.
The crystal structure of the estrogen receptor DNA-binding domain bound to DNA: how receptors discriminate between their response elements.
Cell(Cambridge,Mass.), 75:567-578, 1993

PubMed Abstract: The nuclear hormone receptors are a superfamily of ligand-activated DNA-binding transcription factors. We have determined the crystal structure (at 2.4 A) of the fully specific complex between the DNA-binding domain from the estrogen receptor and DNA. The protein binds as a symmetrical dimer to its palindromic binding site consisting of two 6 bp consensus half sites with three intervening base pairs. This structure reveals how the protein recognizes its own half site sequence rather than that of the related glucocorticoid receptor, which differs by only two base pairs. Since all nuclear hormone receptors recognize one or the other of these two consensus half site sequences, this recognition mechanism applies generally to the whole receptor family.

PubMed: 8221895
DOI: 10.1016/0092-8674(93)90390-C

Experimental method

X-RAY DIFFRACTION (2.4 Å)