1HCQ

THE CRYSTAL STRUCTURE OF THE ESTROGEN RECEPTOR DNA-BINDING DOMAIN BOUND TO DNA: HOW RECEPTORS DISCRIMINATE BETWEEN THEIR RESPONSE ELEMENTS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Spacegroup name	P 21 21 21
Unit cell lengths	51.600, 90.800, 114.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.400
R-factor	0.204
Rwork	0.204
RMSD bond length	0.018 *
RMSD bond angle	2.550 *
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.107 *	0.402 *
Total number of observations	98492 *
Number of reflections	20360 *
Completeness [%]	93.9 *	87.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	DNA duplex	0.07 (mM)
10	1	reservoir	methyl pentane	8 (%)	or ethanol
2	1	drop	440-525	0.14 (mM)
3	1	drop		1.8 (mM)
4	1	drop		25 (mM)
5	1	drop	MES
6	1	drop	spermine	1.8 (mM)
7	1	drop		0.002 (mM)
8	1	reservoir		0.2 (M)
9	1	reservoir	MES