1GTF

The structure of the trp RNA-binding attenuation protein (TRAP) bound to a 53-nucleotide RNA molecule containing GAGUU repeats

Summary for 1GTF

• Entry DOI: 10.2210/pdb1gtf/pdb
• Related: 1C9S 1QAW
• NMR Information: BMRB: 5403
• Descriptor: TRP RNA-BINDING ATTENUATION PROTEIN (TRAP), (GAGUU)10GAG 53-NUCLEOTIDE RNA, TRYPTOPHAN, ... (4 entities in total)
• Functional Keywords: rna binding protein-rna complex, transcription attenuation, rna-binding protein, trp rna, rna binding protein/rna
• Biological source: BACILLUS STEAROTHERMOPHILUS; More
• Total number of polymer chains: 23
• Total formula weight: 204061.72

Authors

Hopcroft, N.H.,Wendt, A.L.,Gollnick, P.,Antson, A.A. (deposition date: 2002-01-15, release date: 2002-04-05, Last modification date: 2023-12-13)

Primary citation

Hopcroft, N.H.,Wendt, A.L.,Gollnick, P.,Antson, A.A.
Specificity of Trap-RNA Interactions: Crystal Structures of Two Complexes with Different RNA Sequences
Acta Crystallogr.,Sect.D, 58:615-, 2002

PubMed Abstract: The trp RNA-binding attenuation protein (TRAP) regulates expression of the tryptophan biosynthetic genes in bacilli by binding to the leader region of the nascent trp operon mRNA. When activated by binding tryptophan, the 11-subunit circular TRAP molecule binds to a target sequence consisting of 11 (G/U)AG repeats, separated by two or three variable 'spacer' nucleotides. Reported here are two crystal structures of TRAP bound to RNAs containing 11 GAG repeats separated by UU and CC spacer nucleotides, determined at 1.75 and 2.50 A resolution, respectively. These show the spacer regions of the RNA molecules to be highly flexible, making no direct hydrogen-bonding contacts with the protein. Comparison of these structures with the previous structure of TRAP bound to (GAGAU)(10)GAG RNA, in which the spacer nucleotides stack with each other close to the protein surface, shows that the RNA can adopt different conformations depending on the sequence of the spacer regions. This gives insight into the structural basis of the specificity of TRAP and into the mechanism of binding.

PubMed: 11914485
DOI: 10.1107/S0907444902003189

Experimental method

X-RAY DIFFRACTION (1.75 Å)