1GTF

The structure of the trp RNA-binding attenuation protein (TRAP) bound to a 53-nucleotide RNA molecule containing GAGUU repeats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	120
Collection date	1999-02-15
Spacegroup name	C 1 2 1
Unit cell lengths	142.077, 111.493, 138.232
Unit cell angles	90.00, 117.28, 90.00

Refinement procedure

Resolution	50.000 * - 1.750
R-factor	0.195 *
Rwork	0.194
R-free	0.24500 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1c9s
RMSD bond length	0.021 *
RMSD bond angle	2.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.07)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.073	0.450
Number of reflections	184884 *	7925 *
<I/σ(I)>	15	1.7
Completeness [%]	97.6 *	82.6
Redundancy	3	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.8 *		0.2M K-GLUTAMATE, 50 MM TRIETHANOLAMINE PH8.0, 10MM MGCL2, 8-11% MONOMETHYL ETHER PEG 2000 + 0.4M KCL AT END, pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein-RNA	15 (mg/ml)
2	1	drop	potassium phosphate	70 (mM)	pH7.8
3	1	drop	L-tryptophan	10 (mM)
4	1	reservoir	potassium glutamate	0.2 (M)
5	1	reservoir	tri-ethanolamine	50 (mM)	pH8.0
6	1	reservoir		10 (mM)
7	1	reservoir	PEG2000 MME	8-11 (%)