1FS1

INSIGHTS INTO SCF UBIQUITIN LIGASES FROM THE STRUCTURE OF THE SKP1-SKP2 COMPLEX

1FS1 の概要

• エントリーDOI: 10.2210/pdb1fs1/pdb
• 関連するPDBエントリー: 1fqv 1fs2
• 分子名称: CYCLIN A/CDK2-ASSOCIATED P19, CYCLIN A/CDK2-ASSOCIATED P45 (3 entities in total)
• 機能のキーワード: skp1, skp2, f-box, lrr, leucine-rich repeat, scf, ubiquitin, e3, ubiquitin protein ligase, ligase
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 44236.59

構造登録者

Schulman, B.A.,Carrano, A.C.,Jeffrey, P.D.,Bowen, Z.,Kinnucan, E.R.E.,Finnin, M.S.,Elledge, S.J.,Harper, J.W.,Pagano, M.,Pavletich, N.P. (登録日: 2000-09-08, 公開日: 2000-11-29, 最終更新日: 2024-02-07)

主引用文献

Schulman, B.A.,Carrano, A.C.,Jeffrey, P.D.,Bowen, Z.,Kinnucan, E.R.,Finnin, M.S.,Elledge, S.J.,Harper, J.W.,Pagano, M.,Pavletich, N.P.
Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex.
Nature, 408:381-386, 2000

PubMed Abstract: F-box proteins are members of a large family that regulates the cell cycle, the immune response, signalling cascades and developmental programmes by targeting proteins, such as cyclins, cyclin-dependent kinase inhibitors, IkappaBalpha and beta-catenin, for ubiquitination (reviewed in refs 1-3). F-box proteins are the substrate-recognition components of SCF (Skp1-Cullin-F-box protein) ubiquitin-protein ligases. They bind the SCF constant catalytic core by means of the F-box motif interacting with Skp1, and they bind substrates through their variable protein-protein interaction domains. The large number of F-box proteins is thought to allow ubiquitination of numerous, diverse substrates. Most organisms have several Skp1 family members, but the function of these Skp1 homologues and the rules of recognition between different F-box and Skp1 proteins remain unknown. Here we describe the crystal structure of the human F-box protein Skp2 bound to Skp1. Skp1 recruits the F-box protein through a bipartite interface involving both the F-box and the substrate-recognition domain. The structure raises the possibility that different Skp1 family members evolved to function with different subsets of F-box proteins, and suggests that the F-box protein may not only recruit substrate, but may also position it optimally for the ubiquitination reaction.

PubMed: 11099048
DOI: 10.1038/35042620

実験手法

X-RAY DIFFRACTION (1.8 Å)