1C82
MECHANISM OF HYALURONAN BINDING AND DEGRADATION: STRUCTURE OF STREPTOCOCCUS PNEUMONIAE HYALURONATE LYASE IN COMPLEX WITH HYALURONIC ACID DISACCHARIDE AT 1.7 A RESOLUTION
Summary for 1C82
| Entry DOI | 10.2210/pdb1c82/pdb |
| Descriptor | HYALURONATE LYASE, 4-deoxy-alpha-L-threo-hex-4-enopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose, CACODYLATE ION, ... (5 entities in total) |
| Functional Keywords | protein-carbohydrate complex, lyase |
| Biological source | Streptococcus pneumoniae |
| Cellular location | Secreted, cell wall; Peptidoglycan-anchor (Potential): Q54873 |
| Total number of polymer chains | 1 |
| Total formula weight | 84640.53 |
| Authors | Ponnuraj, K.,Jedrzejas, M.J. (deposition date: 2000-04-05, release date: 2001-04-05, Last modification date: 2023-12-27) |
| Primary citation | Ponnuraj, K.,Jedrzejas, M.J. Mechanism of hyaluronan binding and degradation: structure of Streptococcus pneumoniae hyaluronate lyase in complex with hyaluronic acid disaccharide at 1.7 A resolution. J.Mol.Biol., 299:885-895, 2000 Cited by PubMed Abstract: Hyaluronic acid (HA) is an important constituent of the extracellular matrix; its bacterial degradation has been postulated to contribute to the spread of certain streptococci through tissue. Pneumococci and other streptococci produce hyaluronate lyase, an enzyme which depolymerizes HA, thus hyaluronate lyase might contribute directly to bacterial invasion. Although two different mechanisms for lyase action have been proposed, there was no crystallographic evidence to support those mechanisms. Here, we report the high-resolution crystal structure of Streptococcus pneumoniae hyaluronate lyase in the presence of HA disaccharide product, which ultimately provides the first crystallographic evidence for the binding of HA to hyaluronate lyase. This structural complex revealed a key interaction between the Streptococcus peneumoniae hyaluronate lyase protein and the product, and supports our previously proposed novel catalytic mechanism for HA degradation based on the native Streptococcus peneumoniae hyaluronate lyase structure. The information provided by this complex structure will likely be useful in the development of antimicrobial pharmaceutical agents. PubMed: 10843845DOI: 10.1006/jmbi.2000.3817 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.7 Å) |
Structure validation
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