1BJT
TOPOISOMERASE II RESIDUES 409-1201
Summary for 1BJT
| Entry DOI | 10.2210/pdb1bjt/pdb |
| Descriptor | TOPOISOMERASE II (2 entities in total) |
| Functional Keywords | topoisomerase, quaternary change, dna-binding, dna topology |
| Biological source | Saccharomyces cerevisiae (baker's yeast) |
| Cellular location | Nucleus: P06786 |
| Total number of polymer chains | 1 |
| Total formula weight | 91810.60 |
| Authors | Fass, D.,Bogden, C.E.,Berger, J.M. (deposition date: 1998-06-29, release date: 1999-05-04, Last modification date: 2024-02-07) |
| Primary citation | Fass, D.,Bogden, C.E.,Berger, J.M. Quaternary changes in topoisomerase II may direct orthogonal movement of two DNA strands. Nat.Struct.Biol., 6:322-326, 1999 Cited by PubMed Abstract: Type II DNA topoisomerases mediate the passage of one DNA duplex through a transient break in another, an event essential for chromosome segregation and cell viability. The active sites of the type II topoisomerase dimer associate covalently with the DNA break-points and must separate by at least the width of the second DNA duplex to accommodate transport. A new structure of the Saccharomyces cerevisiae topoisomerase II DNA-binding and cleavage core suggests that in addition to conformational changes in the DNA-opening platform, a dramatic reorganization of accessory domains may occur during catalysis. These conformational differences have implications for both the DNA-breaking and duplex-transport events in the topo II reaction mechanism, suggest a mechanism by which two distinct drug-resistance loci interact, and illustrate the scope of structural changes in the cycling of molecular machines. PubMed: 10201398DOI: 10.1038/7556 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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