1BJT
TOPOISOMERASE II RESIDUES 409-1201
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 118 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03 |
| Detector | FUJI |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 103.400, 145.700, 161.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.000 - 2.500 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26900 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.164 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.060 | 0.206 |
| Number of reflections | 41611 | |
| <I/σ(I)> | 29.8 | 4.5 |
| Completeness [%] | 97.6 | 95 |
| Redundancy | 3.5 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 4 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG3350 | 1-2 (%) | |
| 2 | 1 | drop | Tris-Cl | 20-40 (mM) | |
| 3 | 1 | reservoir | PEG3350 | 1-2 (%) | |
| 4 | 1 | reservoir | ammonium acetate | 250-300 (mM) | |
| 5 | 1 | reservoir | Tris-Cl | 20-40 (mM) |
