1BJT
TOPOISOMERASE II RESIDUES 409-1201
Experimental procedure
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 118 |
Detector technology | IMAGE PLATE |
Collection date | 1997-03 |
Detector | FUJI |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 103.400, 145.700, 161.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.000 - 2.500 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.26900 |
Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.164 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR (3.851) |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.060 | 0.206 |
Number of reflections | 41611 | |
<I/σ(I)> | 29.8 | 4.5 |
Completeness [%] | 97.6 | 95 |
Redundancy | 3.5 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8 | 4 * | pH 8.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | PEG3350 | 1-2 (%) | |
2 | 1 | drop | Tris-Cl | 20-40 (mM) | |
3 | 1 | reservoir | PEG3350 | 1-2 (%) | |
4 | 1 | reservoir | ammonium acetate | 250-300 (mM) | |
5 | 1 | reservoir | Tris-Cl | 20-40 (mM) |