4V4F
The structure of the trp RNA-binding attenuation protein (TRAP) bound to a RNA molecule containing UAGAU repeats
This is a non-PDB format compatible entry.
Summary for 4V4F
| Entry DOI | 10.2210/pdb4v4f/pdb |
| Related | 1C9S 1GTF 1GTN 1QAW 1UTD |
| Descriptor | 5'-R(*UP*AP*GP*AP*UP)-3', TRANSCRIPTION ATTENUATION PROTEIN MTRB, TRYPTOPHAN, ... (4 entities in total) |
| Functional Keywords | rna binding protein, transcription attenuation, rna-binding protein, trp rna |
| Biological source | BACILLUS STEAROTHERMOPHILUS More |
| Total number of polymer chains | 66 |
| Total formula weight | 406872.31 |
| Authors | Hopcroft, N.H.,Manfredo, A.,Wendt, A.L.,Brzozowski, A.M.,Gollnick, P.,Antson, A.A. (deposition date: 2003-12-08, release date: 2014-07-09, Last modification date: 2024-01-10) |
| Primary citation | Hopcroft, N.H.,Manfredo, A.,Wendt, A.L.,Brzozowski, A.M.,Gollnick, P.,Antson, A.A. The Interaction of RNA with Trap: The Role of Triplet Repeats and Separating Spacer Nucleotides J.Mol.Biol., 338:43-, 2004 Cited by PubMed Abstract: The trp RNA-binding attenuation protein (TRAP) regulates expression of the tryptophan biosynthetic genes in several Bacilli by binding to the leader region of the nascent trp mRNA, inhibiting continued transcription. The 11 subunit TRAP molecule is active in complex with tryptophan, and binds consequently an RNA target segment consisting of 11 (G/U)AG triplets, each separated by two or three non-conserved "spacer" nucleotides. Here, we report the first crystal structures of TRAP in a complex with RNA containing UAG triplets separated by two nucleotides and in a complex with RNA containing GAG triplets separated by three nucleotides. Comparison with known structures of TRAP-RNA complexes shows that both substitution of G-1 with U-1 in the triplet and addition of an extra spacer nucleotide lead to a more flexible complex. This suggests an explanation why, in the trp leader RNA, all three-nucleotide spacer regions are followed by a G-1 nucleotide. Taken together, the structures demonstrate that RNA binding to TRAP is mediated by specific interactions involving the A-2 and G-3 nucleotides of the triplet. This is accompanied by the disruption of stacking interactions between the bases of the other nucleotides, contributing to the increase in entropy that drives binding. PubMed: 15050822DOI: 10.1016/J.JMB.2004.02.038 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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