4V4F
The structure of the trp RNA-binding attenuation protein (TRAP) bound to a RNA molecule containing UAGAU repeats
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 120 |
| Collection date | 2002-06-15 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.907, 133.986, 232.826 |
| Unit cell angles | 90.00, 100.11, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c9s |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.422 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.056 | 0.284 |
| Number of reflections | 251906 | |
| <I/σ(I)> | 19.3 | 2.7 |
| Completeness [%] | 92.2 | 58 |
| Redundancy | 3.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.2M K-GLUTAMATE, 50 MM TRIETHANOLAMINE PH8.0, 10MM MGCL2, 8-11% MONOMETHYL ETHER PEG 2000, +0.4M KCL AT END, pH 8.00 |
